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feng zhang  (Sino Biological)


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    Structured Review

    Sino Biological feng zhang
    Feng Zhang, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/g2e3+flag+plasmids/Human+G2E3+Gene+ORF+cDNA+clone+expression+plasmid%2C+C-Flag+tag/pm41068090-167-58-68
    Average 93 stars, based on 1 article reviews
    feng zhang - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progression.
    Article Snippet: .. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http:// n2t.net/addgene:12259; RRID:Addgene_12259), psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID: Addgene_12260), FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060; http://n2t.net/addgene:110060; RRID:Addgene_110060), Ubiquitination-Related Proteins CRISPR Knockout Library (Addgene #174592; http://n2t.net/addgene:174592; RRID:Addgene_174592), pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988), G2E3-FLAG plasmids (Sino Biological Cat# HG22847-CF) and FLAG plasmids (Sino Biological Cat# CV012) were provided by Sino Biological. .. HEK293TN cells (BioCat cat. # LV900A-1-GVO-SBI, RRID: CVCL_UL49) were grown in DMEM medium containing 10% inactivated fetal bovine serum (FBS) (GibcoTM Cat# A3160501).

    Article Title: CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progression
    Article Snippet: For immunofluorescence, anti-LC3B (Cell Signaling Technology Cat# 3868, RRID:AB_2137707), anti-mouse IgG Alexa Fluor® 555 conjugate (Cell Signaling Technology Cat# 4409, RRID:AB_1904022), anti-rabbit Alexa Fluor® 555 conjugate (Cell Signaling Technology Cat# 19581, RRID:AB_2943221), anti-rabbit IgG Alexa Fluor® 488 conjugate (Cell Signaling Technology Cat# 4412, RRID:AB_1904025), anti-mouse IgG Alexa Fluor® 488 conjugate (Cell Signaling Technology Cat# 4523, RRID:AB_836889) were purchased from Cell Signaling Technology. .. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259), psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260 ; RRID: Addgene_12260), FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060; http://n2t.net/addgene:110060 ; RRID:Addgene_110060), Ubiquitination-Related Proteins CRISPR Knockout Library (Addgene #174592; http://n2t.net/addgene:174592 ; RRID:Addgene_174592), pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988 ; RRID:Addgene_62988), G2E3-FLAG plasmids (Sino Biological Cat# HG22847-CF) and FLAG plasmids (Sino Biological Cat# CV012) were provided by Sino Biological. .. HEK293TN cells (BioCat cat. # LV900A-1-GVO-SBI, RRID: CVCL_UL49) were grown in DMEM medium containing 10% inactivated fetal bovine serum (FBS) (GibcoTM Cat# A3160501).

    Ubiquitin Proteomics:

    Article Title: CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progression.
    Article Snippet: .. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http:// n2t.net/addgene:12259; RRID:Addgene_12259), psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID: Addgene_12260), FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060; http://n2t.net/addgene:110060; RRID:Addgene_110060), Ubiquitination-Related Proteins CRISPR Knockout Library (Addgene #174592; http://n2t.net/addgene:174592; RRID:Addgene_174592), pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988), G2E3-FLAG plasmids (Sino Biological Cat# HG22847-CF) and FLAG plasmids (Sino Biological Cat# CV012) were provided by Sino Biological. .. HEK293TN cells (BioCat cat. # LV900A-1-GVO-SBI, RRID: CVCL_UL49) were grown in DMEM medium containing 10% inactivated fetal bovine serum (FBS) (GibcoTM Cat# A3160501).

    Article Title: CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progression
    Article Snippet: For immunofluorescence, anti-LC3B (Cell Signaling Technology Cat# 3868, RRID:AB_2137707), anti-mouse IgG Alexa Fluor® 555 conjugate (Cell Signaling Technology Cat# 4409, RRID:AB_1904022), anti-rabbit Alexa Fluor® 555 conjugate (Cell Signaling Technology Cat# 19581, RRID:AB_2943221), anti-rabbit IgG Alexa Fluor® 488 conjugate (Cell Signaling Technology Cat# 4412, RRID:AB_1904025), anti-mouse IgG Alexa Fluor® 488 conjugate (Cell Signaling Technology Cat# 4523, RRID:AB_836889) were purchased from Cell Signaling Technology. .. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259), psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260 ; RRID: Addgene_12260), FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060; http://n2t.net/addgene:110060 ; RRID:Addgene_110060), Ubiquitination-Related Proteins CRISPR Knockout Library (Addgene #174592; http://n2t.net/addgene:174592 ; RRID:Addgene_174592), pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988 ; RRID:Addgene_62988), G2E3-FLAG plasmids (Sino Biological Cat# HG22847-CF) and FLAG plasmids (Sino Biological Cat# CV012) were provided by Sino Biological. .. HEK293TN cells (BioCat cat. # LV900A-1-GVO-SBI, RRID: CVCL_UL49) were grown in DMEM medium containing 10% inactivated fetal bovine serum (FBS) (GibcoTM Cat# A3160501).

    CRISPR:

    Article Title: CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progression.
    Article Snippet: .. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http:// n2t.net/addgene:12259; RRID:Addgene_12259), psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID: Addgene_12260), FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060; http://n2t.net/addgene:110060; RRID:Addgene_110060), Ubiquitination-Related Proteins CRISPR Knockout Library (Addgene #174592; http://n2t.net/addgene:174592; RRID:Addgene_174592), pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988), G2E3-FLAG plasmids (Sino Biological Cat# HG22847-CF) and FLAG plasmids (Sino Biological Cat# CV012) were provided by Sino Biological. .. HEK293TN cells (BioCat cat. # LV900A-1-GVO-SBI, RRID: CVCL_UL49) were grown in DMEM medium containing 10% inactivated fetal bovine serum (FBS) (GibcoTM Cat# A3160501).

    Article Title: CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progression
    Article Snippet: For immunofluorescence, anti-LC3B (Cell Signaling Technology Cat# 3868, RRID:AB_2137707), anti-mouse IgG Alexa Fluor® 555 conjugate (Cell Signaling Technology Cat# 4409, RRID:AB_1904022), anti-rabbit Alexa Fluor® 555 conjugate (Cell Signaling Technology Cat# 19581, RRID:AB_2943221), anti-rabbit IgG Alexa Fluor® 488 conjugate (Cell Signaling Technology Cat# 4412, RRID:AB_1904025), anti-mouse IgG Alexa Fluor® 488 conjugate (Cell Signaling Technology Cat# 4523, RRID:AB_836889) were purchased from Cell Signaling Technology. .. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259), psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260 ; RRID: Addgene_12260), FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060; http://n2t.net/addgene:110060 ; RRID:Addgene_110060), Ubiquitination-Related Proteins CRISPR Knockout Library (Addgene #174592; http://n2t.net/addgene:174592 ; RRID:Addgene_174592), pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988 ; RRID:Addgene_62988), G2E3-FLAG plasmids (Sino Biological Cat# HG22847-CF) and FLAG plasmids (Sino Biological Cat# CV012) were provided by Sino Biological. .. HEK293TN cells (BioCat cat. # LV900A-1-GVO-SBI, RRID: CVCL_UL49) were grown in DMEM medium containing 10% inactivated fetal bovine serum (FBS) (GibcoTM Cat# A3160501).

    Knock-Out:

    Article Title: CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progression.
    Article Snippet: .. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http:// n2t.net/addgene:12259; RRID:Addgene_12259), psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260; RRID: Addgene_12260), FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060; http://n2t.net/addgene:110060; RRID:Addgene_110060), Ubiquitination-Related Proteins CRISPR Knockout Library (Addgene #174592; http://n2t.net/addgene:174592; RRID:Addgene_174592), pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988; RRID:Addgene_62988), G2E3-FLAG plasmids (Sino Biological Cat# HG22847-CF) and FLAG plasmids (Sino Biological Cat# CV012) were provided by Sino Biological. .. HEK293TN cells (BioCat cat. # LV900A-1-GVO-SBI, RRID: CVCL_UL49) were grown in DMEM medium containing 10% inactivated fetal bovine serum (FBS) (GibcoTM Cat# A3160501).

    Article Title: CRISPR-Cas9 screening reveals G2E3 as a novel ubiquitin-linked factor controlling autophagosome-lysosome fusion and cancer cell progression
    Article Snippet: For immunofluorescence, anti-LC3B (Cell Signaling Technology Cat# 3868, RRID:AB_2137707), anti-mouse IgG Alexa Fluor® 555 conjugate (Cell Signaling Technology Cat# 4409, RRID:AB_1904022), anti-rabbit Alexa Fluor® 555 conjugate (Cell Signaling Technology Cat# 19581, RRID:AB_2943221), anti-rabbit IgG Alexa Fluor® 488 conjugate (Cell Signaling Technology Cat# 4412, RRID:AB_1904025), anti-mouse IgG Alexa Fluor® 488 conjugate (Cell Signaling Technology Cat# 4523, RRID:AB_836889) were purchased from Cell Signaling Technology. .. pMD2.G was a gift from Didier Trono (Addgene plasmid # 12259; http://n2t.net/addgene:12259 ; RRID:Addgene_12259), psPAX2 was a gift from Didier Trono (Addgene plasmid # 12260; http://n2t.net/addgene:12260 ; RRID: Addgene_12260), FUW mCherry-GFP-LC3 was a gift from Anne Brunet (Addgene plasmid # 110060; http://n2t.net/addgene:110060 ; RRID:Addgene_110060), Ubiquitination-Related Proteins CRISPR Knockout Library (Addgene #174592; http://n2t.net/addgene:174592 ; RRID:Addgene_174592), pSpCas9(BB)-2A-Puro (PX459) V2.0 was a gift from Feng Zhang (Addgene plasmid # 62988; http://n2t.net/addgene:62988 ; RRID:Addgene_62988), G2E3-FLAG plasmids (Sino Biological Cat# HG22847-CF) and FLAG plasmids (Sino Biological Cat# CV012) were provided by Sino Biological. .. HEK293TN cells (BioCat cat. # LV900A-1-GVO-SBI, RRID: CVCL_UL49) were grown in DMEM medium containing 10% inactivated fetal bovine serum (FBS) (GibcoTM Cat# A3160501).



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